Journal: Oncotarget

Article Title: Local application of bacteria improves safety of Salmonella -mediated tumor therapy and retains advantages of systemic infection

doi: 10.18632/oncotarget.18392

Figure Lengend Snippet: CT26 tumor-bearing mice were infected with 5×10 6 CFU SF200 ( ΔlpxR9 ΔpagL7 ΔpagP8 ΔaroA ΔydiV ΔfliF ) (A) , Symbioflor-2 (C) and E. coli Nissle (D) . Considering more resilient tumors, F1.A11 tumor-bearing mice were infected with 5×10 6 SF200 (B) . Straight lines depict i.v. infection and dotted lines i.t. infection. Tumor volumes were calculated on the basis of caliper measurements. PBS served as a negative control. Displayed are values of mean ± SEM. Results are representative of two independent experiments with five replicates in each group.

Article Snippet: Six to seven week old BALB/c mice (Janvier) were intradermally inoculated with 5×10 5 syngeneic CT26 tumor cells (colorectal cancer, ATCC CRL-2638) or 5×10 5 F1.A11 tumor cells (fibrosarcoma) in the right flank.

Techniques: Infection, Negative Control

Journal: Oncotarget

Article Title: Local application of bacteria improves safety of Salmonella -mediated tumor therapy and retains advantages of systemic infection

doi: 10.18632/oncotarget.18392

Figure Lengend Snippet: CT26 tumor-bearing mice were infected with 5×10 6 CFU SF200 ( ΔlpxR9 ΔpagL7 ΔpagP8 ΔaroA ΔydiV ΔfliF ) via i.t. and i.v. routes of inoculation (top and bottom row, resp.). 48 hpi, tumors were isolated, embedded in paraffin and prepared for immune histochemical staining. Similar histological profiles between i.t. and i.v. infections: similar degree of necrosis formation and hypoxia, dispersion of salmonellae in and beyond necrotic center, and presence of neutrophils in immediate proximity to the salmonellae. Images displayed are representative of four replicates. “N” denotes areas of necrosis. Hypoxia was stained with antibodies against metabolites of pimonidazole-HCl administered i.v. 30 mins prior to isolation. Myeloperoxidase (MPO) denotes presence of neutrophilic granulocytes, and salmonellae was stained using a specific antibody. Differential staining was performed on consecutive sections. Scale bar corresponds to 100 μm. Images representative of at least 3 replicates are displayed.

Article Snippet: Six to seven week old BALB/c mice (Janvier) were intradermally inoculated with 5×10 5 syngeneic CT26 tumor cells (colorectal cancer, ATCC CRL-2638) or 5×10 5 F1.A11 tumor cells (fibrosarcoma) in the right flank.

Techniques: Infection, Isolation, Staining, Dispersion

Journal: Oncotarget

Article Title: Local application of bacteria improves safety of Salmonella -mediated tumor therapy and retains advantages of systemic infection

doi: 10.18632/oncotarget.18392

Figure Lengend Snippet: CT26 tumor bearing mice were infected i.v. and i.t. with 5×10 6 CFU SF200 ( ΔlpxR9 ΔpagL7 ΔpagP8 ΔaroA ΔydiV ΔfliF ) and probiotic E. coli . (A-C) Blood, spleen, liver and tumor were analyzed for bacterial burden by plating serial dilutions of tissue homogenates. CFU counts were determined 48 hpi. Significantly lower numbers were observed during i.t. infections. (D-F) Body weight as indicator for the general health status of mice. Again, i.t. infection resulted in reduced body weight loss. PBS served as negative control. Displayed are medians with range. Results are representative of two independent experiments with five replicates per group. *, p<0.05; **, p<0.01; ***, p<0.001.

Article Snippet: Six to seven week old BALB/c mice (Janvier) were intradermally inoculated with 5×10 5 syngeneic CT26 tumor cells (colorectal cancer, ATCC CRL-2638) or 5×10 5 F1.A11 tumor cells (fibrosarcoma) in the right flank.

Techniques: Infection, Negative Control

Journal: Oncotarget

Article Title: Local application of bacteria improves safety of Salmonella -mediated tumor therapy and retains advantages of systemic infection

doi: 10.18632/oncotarget.18392

Figure Lengend Snippet: CT26- tumor bearing mice were infected i.v. and i.t. with a dose of 5×10 6 and 5×10 5 Salmonella variant SF201 ( ΔlpxR9 ΔpagL7 ΔpagP8 ΔaroA ΔssrA: :Km). Tumors (A) , blood (B) , spleen (C) and liver (D) . Bacterial burden was determined by plating serial dilutions of tissue homogenates. CFU were analyzed 36 hpi. (E) Tumor volumes were calculated on the basis of caliper measurements following infection with SF201. PBS served as negative control. (F) Body weight measurement served as indicator for the general health status. I.t. infection resulted in a significantly reduced bacterial burden and body weight decrease. PBS served as negative control. Displayed are medians with range. Results are representative of two independent experiments with five replicates per group.

Article Snippet: Six to seven week old BALB/c mice (Janvier) were intradermally inoculated with 5×10 5 syngeneic CT26 tumor cells (colorectal cancer, ATCC CRL-2638) or 5×10 5 F1.A11 tumor cells (fibrosarcoma) in the right flank.

Techniques: Infection, Variant Assay, Negative Control

Journal: Oncotarget

Article Title: Local application of bacteria improves safety of Salmonella -mediated tumor therapy and retains advantages of systemic infection

doi: 10.18632/oncotarget.18392

Figure Lengend Snippet: (A) TNF-α levels in sera of CT26 tumor bearing mice isolated 1.5 h after infection with SF200 ( ΔlpxR9 ΔpagL7 ΔpagP8 ΔaroA ΔydiV ΔfliF ). (B) CT26 tumor development in Rag1 −/− mice reconstituted with CD8 + T cells at the time of CT26 inoculation. 3×10 6 CD8 + T cells were adoptively transferred from uninfected CT26 bearing mice (“CD8 + T cells (CT26)”) or CT26 tumor bearing mice treated with SF200 ((“CD8 + T cells (Infection)”). PBS served as negative control. (C) Endpoint tumor volume at day 14 post transfer. Displayed are values of mean ± SEM. Results are representative of two independent experiments with five replicates per group. *, p<0.05; **, p<0.01; ***, p<0.001.

Article Snippet: Six to seven week old BALB/c mice (Janvier) were intradermally inoculated with 5×10 5 syngeneic CT26 tumor cells (colorectal cancer, ATCC CRL-2638) or 5×10 5 F1.A11 tumor cells (fibrosarcoma) in the right flank.

Techniques: Isolation, Infection, Negative Control

Journal: Oncotarget

Article Title: Local application of bacteria improves safety of Salmonella -mediated tumor therapy and retains advantages of systemic infection

doi: 10.18632/oncotarget.18392

Figure Lengend Snippet: (A) Mice bearing two CT26 tumors were infected i.v. or i.t. into one tumor with 5×10 6 CFU SF202 ( ΔlpxR9 ΔpagL7 ΔpagP8 ΔaroA ΔydiV ΔfliF pHL304). Anesthetized mice were analyzed at 0, 1, 2 and 3 dpi using a non-invasive in vivo imaging system (IVIS200). (B) Bacterial colonization of tumors was determined by plating serial dilutions of tissue homogenates. CFU were analyzed 12, 24, 48 and 72 hpi to match imaging time points. For i.t. infection, primary and secondary tumors are denoted 1° and 2°, respectively. Displayed are medians with range. Results are representative of two independent experiments with five replicates in each group.

Article Snippet: Six to seven week old BALB/c mice (Janvier) were intradermally inoculated with 5×10 5 syngeneic CT26 tumor cells (colorectal cancer, ATCC CRL-2638) or 5×10 5 F1.A11 tumor cells (fibrosarcoma) in the right flank.

Techniques: Infection, In Vivo Imaging, Imaging